ACE2 Recombinant Antibody

Catalogue Number: BSM-52614R-BSS

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BSM-52614R Image

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Human kidney Probed with Angiotensin Converting Enzyme 2 (3F1) Monoclonal Antibody (bsm-52614R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

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IF(ICC) staining with Angiotensin Converting Enzyme 2 (3F1) Monoclonal Antibody (bsm-52614R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

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IF(ICC) staining with Angiotensin Converting Enzyme 2 (3F1) Monoclonal Antibody (bsm-52614R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

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Paraformaldehyde-fixed and paraffin-embedded human breast carcinoma tissue incubated with Angiotensin Converting Enzyme 2 (3F1) Monoclonal Antibody (bsm-52614R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

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Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with Angiotensin Converting Enzyme 2 (3F1) Monoclonal Antibody (bsm-52614R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

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Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with Angiotensin Converting Enzyme 2 (3F1) Monoclonal Antibody (bsm-52614R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

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Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Angiotensin Converting Enzyme 2 (3F1) Monoclonal Antibody, Unconjugated (bsm-52614R) at 1:200 overnight at 4°C, DAB staining.

bsm-52614R Image

Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Angiotensin Converting Enzyme 2 (3F1) Monoclonal Antibody, Unconjugated (bsm-52614R) at 1:200 overnight at 4°C, DAB staining.

bsm-52614R Image

Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Angiotensin Converting Enzyme 2 (3F1) Monoclonal Antibody, Unconjugated (bsm-52614R) at 1:200 overnight at 4°C, DAB staining.

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Lane 1: Recombinant human ACE2 protein, His & Avi (HEK293)( bs-46001P) probed with ACE2 Monoclonal Antibody, Unconjugated (bsm-52614R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

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Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ACE2 Monoclonal Antibody, Unconjugated(bsm-52614R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-52614R Image

Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ACE2 Monoclonal Antibody, Unconjugated(bsm-52614R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

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25 ug total protein per lane of various lysates (see on figure) probed with ACE2 monoclonal antibody, unconjugated (bsm-52614R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

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Manufacturer:	Bioss Inc
Shelf Life:	12 months
Type:	Recombinant Monoclonal
Alias:	Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, Angiotensin-converting enzyme homolog, Metalloprotease MPROT15, ACEH, ACE2
Shipping Condition:	Blue Ice
Unit(s):	100 ul, 20 ul
Host name:	Rabbit
Clone:
Isotype:	IgG
Immunogen:	C terminal human Angiotensin Converting Enzyme 2
Application:	ICC, IF, IHC-P, WB, IHC-Fr

Overview
Datasheets

Description

Description: Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.