CD3D Recombinant Antibody
Catalogue Number: BSM-52744R-BSS
BSM-52744R Image
BSM-52744R Image
BSM-52744R Image
BSM-52744R Image
BSM-52744R Image
BSM-52744R Image
BSM-52744R Image
BSM-52744R Image
BSM-52744R Image
BSM-52744R Image
BSM-52744R Image
Flow cytometric analysis of Raji cells with CD3D (5C1) Monoclonal Antibody (bsm-52744R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
BSM-52744R Image
Jurkat cell lysate probed with CD3D (5C1) Monoclonal Antibody (bsm-52744R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
BSM-52744R Image
IF(ICC) staining with CD3D (5C1) Monoclonal Antibody (bsm-52744R) at 1:300 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
BSM-52744R Image
IF(ICC) staining with CD3D (5C1) Monoclonal Antibody (bsm-52744R) at 1:300 in SW480 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
BSM-52744R Image
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with CD3D (5C1) Monoclonal Antibody (bsm-52744R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52744R Image
Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with CD3D (5C1) Monoclonal Antibody (bsm-52744R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52744R Image
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with CD3D (5C1) Monoclonal Antibody (bsm-52744R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-52744R Image
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD3D Monoclonal Antibody, Unconjugated(bsm-52744R) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
BSM-52744R Image
Paraformaldehyde-fixed, paraffin embedded Human spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD3D Monoclonal Antibody, Unconjugated(bsm-52744R) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
BSM-52744R Image
25 ug total protein per lane of various lysates (see on figure) probed with CD3D monoclonal antibody, unconjugated (bsm-52744R) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Recombinant Monoclonal |
| Alias: | T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain, CD_antigen: CD3d, CD3D |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | C terminus human CD3D |
| Application: | FC, ICC, IF, IHC-P, IP, WB, IHC-Fr |
Description
Description: Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells(PubMed:12215456).
Additional Text
Gene ID
915
Gene Name
CD3D
Uniprot ID
P04234
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Modification
Unmodified
Storage Note
Store at -20°C for 12 months.
bsm-52744R Datasheet
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