MMP9 Recombinant Antibody

Catalogue Number: BSM-54040R-BSS

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Lane 1: Rat Spleen; Probed with MMP9 (7D6) Monoclonal Antibody (bsm-54040R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

BSM-54040R Image

IF(ICC) staining with MMP9 (7D6) Monoclonal Antibody (bsm-54040R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

BSM-54040R Image

IF(ICC) staining with MMP9 (7D6) Monoclonal Antibody (bsm-54040R) at 1:100 in SHG-44 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

BSM-54040R Image

Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with MMP9 (7D6) Monoclonal Antibody (bsm-54040R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

BSM-54040R Image

Paraformaldehyde-fixed and paraffin-embedded Mouse Placenta tissue incubated with MMP9 (7D6) Monoclonal Antibody (bsm-54040R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

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Flow cytometric analysis of A431 cells with MMP9 (7D6) Monoclonal Antibody (bsm-54040R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

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Lane 1: Rat Spleen tissue lysates; Lane 2: Rat Lung tissue lysates probed with MMP9 (7D6) Monoclonal Antibody, Unconjugated (bsm-54040R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

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Lane 1: Normal human U-2 OS cell lysates; Lane 2: U-2 OS treated with 200nM TPA for 48 hours lysates; Lane 3: Normal human MDA-MB-231 cell lysates; Lane 4: MDA-MB-231 serum starved overnight, then treated with 200nM TPA for 24 hours probed with MMP9 Monoclonal Antibody, Unconjugated (bsm-54040R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

BSM-54040R Image

Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MMP9 (7D6) Monoclonal Antibody, Unconjugated (bsm-54040R) at 1:200 overnight at 4°C, DAB staining.

BSM-54040R Image

Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MMP9 (7D6) Monoclonal Antibody, Unconjugated (bsm-54040R) at 1:200 overnight at 4°C, DAB staining.

BSM-54040R Image

25 ug total protein per lane of various lysates (see on figure) probed with MMP9 monoclonal antibody, unconjugated (bsm-54040R) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

BSM-54040R Image

U2OS (H) cells were treated with or without TPA (200nM,48h) for 30 min, 25 µg total protein per lane of cell lysates (see on figure) probed with MMP9 monoclonal antibody, unconjugated (bsm-54040R) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

BSM-54040R Image

MDA-MB-231 (H) cells were treated with or without TPA (200nM,24h) for 30 min, 25 µg total protein per lane of cell lysates (see on figure) probed with MMP9 monoclonal antibody, unconjugated (bsm-54040R) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

BSM-54040R Image

Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP9 Monoclonal Antibody, Unconjugated(bsm-54040R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-54040R Image

Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP9 Monoclonal Antibody, Unconjugated(bsm-54040R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-54040R Image

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP9 Monoclonal Antibody, Unconjugated(bsm-54040R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-54040R Image

Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP9 Monoclonal Antibody, Unconjugated(bsm-54040R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-54040R Image

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP9 Monoclonal Antibody, Unconjugated(bsm-54040R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

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Manufacturer:	Bioss Inc
Shelf Life:	12 months
Type:	Recombinant Monoclonal
Alias:	Matrix metalloproteinase-9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, 67 kDa matrix metalloproteinase-9, 82 kDa matrix metalloproteinase-9, MMP-9, GELB, MMP9, CLG4B
Shipping Condition:	Blue Ice
Unit(s):	100 ul, 20 ul
Host name:	Rabbit
Clone:
Isotype:	IgG
Immunogen:	C terminal human MMP9
Application:	IF, IHC-P, WB, IHC-Fr

Overview
Datasheets

Description

Description: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.