MMP9 (7D6) Monoclonal Antibody
Catalogue Number: BSM-54040R-BSS
BSM-54040R Image
BSM-54040R Image
BSM-54040R Image
BSM-54040R Image
BSM-54040R Image
BSM-54040R Image
BSM-54040R Image
BSM-54040R Image
BSM-54040R Image
BSM-54040R Image
BSM-54040R Image
Lane 1: Rat Spleen; Probed with MMP9 (7D6) Monoclonal Antibody (bsm-54040R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
BSM-54040R Image
IF(ICC) staining with MMP9 (7D6) Monoclonal Antibody (bsm-54040R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
BSM-54040R Image
IF(ICC) staining with MMP9 (7D6) Monoclonal Antibody (bsm-54040R) at 1:100 in SHG-44 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
BSM-54040R Image
Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with MMP9 (7D6) Monoclonal Antibody (bsm-54040R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-54040R Image
Paraformaldehyde-fixed and paraffin-embedded Mouse Placenta tissue incubated with MMP9 (7D6) Monoclonal Antibody (bsm-54040R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
BSM-54040R Image
Flow cytometric analysis of A431 cells with MMP9 (7D6) Monoclonal Antibody (bsm-54040R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
BSM-54040R Image
Lane 1: Rat Spleen tissue lysates; Lane 2: Rat Lung tissue lysates probed with MMP9 (7D6) Monoclonal Antibody, Unconjugated (bsm-54040R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
BSM-54040R Image
Lane 1: Normal human U-2 OS cell lysates; Lane 2: U-2 OS treated with 200nM TPA for 48 hours lysates; Lane 3: Normal human MDA-MB-231 cell lysates; Lane 4: MDA-MB-231 serum starved overnight, then treated with 200nM TPA for 24 hours probed with MMP9 Monoclonal Antibody, Unconjugated (bsm-54040R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
BSM-54040R Image
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MMP9 (7D6) Monoclonal Antibody, Unconjugated (bsm-54040R) at 1:200 overnight at 4°C, DAB staining.
BSM-54040R Image
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MMP9 (7D6) Monoclonal Antibody, Unconjugated (bsm-54040R) at 1:200 overnight at 4°C, DAB staining.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Matrix metalloproteinase-9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, 67 kDa matrix metalloproteinase-9, 82 kDa matrix metalloproteinase-9, MMP-9, GELB, MMP9, CLG4B |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | 7D6 |
| Isotype: | IgG |
| Immunogen: | C terminal human MMP9 |
| Application: | FC, ICC, IF, IHC-P, WB |
Description
Description: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
Additional Text
Gene Name
MMP9
Gene ID
4318
Uniprot ID
P14780
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Monoclonal
Modification
Unmodified
Storage Note
Store for 1 year at -20C from date of shipment. Store for 1 year at -20C from date of shipment.
bsm-54040R Datasheet
SUPPORT
outstanding technical support
PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations