GSTP1 Recombinant Antibody
Catalogue Number: BSM-60581R-BSS
BSM-60581R Image
BSM-60581R Image
BSM-60581R Image
BSM-60581R Image
BSM-60581R Image
BSM-60581R Image
BSM-60581R Image
BSM-60581R Image
BSM-60581R Image
BSM-60581R Image
BSM-60581R Image
BSM-60581R Image
Tissue: Human placenta Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60581R
BSM-60581R Image
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: Jurkat, 2: PC-3, 3: MOLT-4, 4: K562 Protein loading quantity: 20 µg Exposure time: 60 s Predicted MW: 23 kDa Observed MW: 23 kDa
BSM-60581R Image
Cell line: K562 Fixative: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for bsm-60581R
BSM-60581R Image
Cell line: HepG2 Fixative: 4% Paraformaldehyde Permeabilization: 90% Methanol Primary Ab dilution: 1:100 Secondary Ab: Goat anti Rabbit IgG Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for bsm-60581R
BSM-60581R Image
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GSTP1) Monoclonal Antibody, Unconjugated (bsm-60581R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
BSM-60581R Image
Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GSTP1) Monoclonal Antibody, Unconjugated (bsm-60581R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
BSM-60581R Image
Lane 1: Human K562 cell Lysates; Lane 2: Human MOLT4 cell Lysates. Probed with GSTP1 monoclonal Antibody, unconjugated (bsm-60581R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
BSM-60581R Image
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (GSTP1) Monoclonal Antibody, Unconjugated (bsm-60581R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
BSM-60581R Image
Paraformaldehyde-fixed, paraffin embedded (Human placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (GSTP1) Monoclonal Antibody, Unconjugated (bsm-60581R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
BSM-60581R Image
Paraformaldehyde-fixed, paraffin embedded (Human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (GSTP1) Monoclonal Antibody, Unconjugated (bsm-60581R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
BSM-60581R Image
Paraformaldehyde-fixed, paraffin embedded (Human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (GSTP1) Monoclonal Antibody, Unconjugated (bsm-60581R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Recombinant Monoclonal |
| Alias: | Glutathione S-Transferase pi; GST-Pi; DFN7; Gst-P1; FAEES3; Fatty Acid Ethyl Ester Synthase III; Glutathione S Transferase Pi; GST3; GSTP1; GST pi; PI; GSTP1_HUMAN. |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human GSTP1 |
| Application: | FC, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq, Jul 2008]
Additional Text
Uniprot ID
P19157
Purification
Protein A purified
Antibody Clonality
Recombinant Monoclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Gene ID
14870; 2950
Gene Name
Gstp1, GSTP1
BSM-60581R Datasheet
SUPPORT
outstanding technical support
PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations