HSD17B1 Recombinant Antibody
Catalogue Number: BSM-60664R-BSS
BSM-60664R Image
BSM-60664R Image
BSM-60664R Image
BSM-60664R Image
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:20000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: Human heart (Negative control), 2: Human placenta Protein loading quantity: 20 µg Exposure time: 30 s Predicted MW: 35 kDa Observed MW: 35 kDa
BSM-60664R Image
Tissue: Human placenta Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:5000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60664R
BSM-60664R Image
Tissue: Human prostatic hyperplasia (Negative tissue) Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:5000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: No staining on bsm-60664R
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Recombinant Monoclonal |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human HSD17B1 |
| Application: | IF, IHC-P, WB, IHC-Fr |
Description
Description: This gene encodes a member of the 17beta-hydroxysteroid dehydrogenase family of short-chain dehydrogenases/reductases. It has a dual function in estrogen activation and androgen inactivation and plays a major role in establishing the estrogen E2 concentration gradient between serum and peripheral tissues. The encoded protein catalyzes the last step in estrogen activation, using NADPH to convert estrogens E1 and E2 and androgens like 4-androstenedione, to testosterone. It has an N-terminal short-chain dehydrogenase domain with a cofactor binding site, and a narrow, hydrophobic C-terminal domain with a steroid substrate binding site. This gene is expressed primarily in the placenta and ovarian granulosa cells, and to a lesser extent, in the endometrium, adipose tissue, and prostate. Polymorphisms in this gene have been linked to breast and prostate cancer. A pseudogene of this gene has been identified. Alternative splicing results in multiple transcript variants.
Additional Text
Gene Name
HSD17B1
Uniprot ID
P14061
Gene ID
3292
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Recombinant Monoclonal
Modification
Unmodified
Specificity
11beta-HSD1; 17beta-HSD1; 17 beta HSD 1; 17 beta hydroxysteroid dehydrogenase type 1; 17_-hydroxysteroid dehydrogenasetype 1 EDH17B2; EDHB17; Estradiol 17 beta dehydrogenase 1; HSD17; Hydroxysteroid (17 beta) dehydrogenase 1; Placental 17 beta hydroxysteroid dehydrogenase; DHB1_HUMAN.
Storage Note
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
BSM-60664R Datasheet
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PRODUCT
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DELIVERY
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