Acetyl-Histone H3.1 (Lys28) Recombinant Antibody

Catalogue Number: BSM-62460R-BSS

BSM-62460R Image

4% Paraformaldehyde-fixed SH-SY5Y(H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Acetyl-Histone H3.1 (Lys28)) monoclonal Antibody, unconjugated (bsm-62460R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

BSM-62460R Image

25 ug total protein per lane of various lysates (see on figure) probed with Acetyl-Histone H3.1 (Lys28) monoclonal antibody, unconjugated (bsm-62460R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

BSM-62460R Image

The HepG2 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-Acetyl-Histone H3.1 (Lys28) antibody (bsm-62460R): 1 µg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-60295G-FITC): 1 µg/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

BSM-62460R Image

Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Acetyl-Histone H3.1 (Lys28) Monoclonal Antibody, Unconjugated(bsm-62460R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-62460R Image

Paraformaldehyde-fixed, paraffin embedded Rat testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Acetyl-Histone H3.1 (Lys28) Monoclonal Antibody, Unconjugated(bsm-62460R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-62460R Image

Paraformaldehyde-fixed, paraffin embedded Rat cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Acetyl-Histone H3.1 (Lys28) Monoclonal Antibody, Unconjugated(bsm-62460R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-62460R Image

Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Acetyl-Histone H3.1 (Lys28) Monoclonal Antibody, Unconjugated(bsm-62460R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-62460R Image

Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Acetyl-Histone H3.1 (Lys28) Monoclonal Antibody, Unconjugated(bsm-62460R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-62460R Image

Paraformaldehyde-fixed, paraffin embedded Mouse testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Acetyl-Histone H3.1 (Lys28) Monoclonal Antibody, Unconjugated(bsm-62460R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-62460R Image

Paraformaldehyde-fixed, paraffin embedded Mouse cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Acetyl-Histone H3.1 (Lys28) Monoclonal Antibody, Unconjugated(bsm-62460R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-62460R Image

Paraformaldehyde-fixed, paraffin embedded Mouse heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Acetyl-Histone H3.1 (Lys28) Monoclonal Antibody, Unconjugated(bsm-62460R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-62460R Image

Paraformaldehyde-fixed, paraffin embedded Mouse thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Acetyl-Histone H3.1 (Lys28) Monoclonal Antibody, Unconjugated(bsm-62460R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-62460R Image

Paraformaldehyde-fixed, paraffin embedded Rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Acetyl-Histone H3.1 (Lys28) Monoclonal Antibody, Unconjugated(bsm-62460R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-62460R Image

Paraformaldehyde-fixed, paraffin embedded Rat thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Acetyl-Histone H3.1 (Lys28) Monoclonal Antibody, Unconjugated(bsm-62460R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-62460R Image

Paraformaldehyde-fixed, paraffin embedded Human glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Acetyl-Histone H3.1 (Lys28) Monoclonal Antibody, Unconjugated(bsm-62460R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-62460R Image

Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Acetyl-Histone H3.1 (Lys28) Monoclonal Antibody, Unconjugated(bsm-62460R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-62460R Image

Paraformaldehyde-fixed, paraffin embedded Human testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Acetyl-Histone H3.1 (Lys28) Monoclonal Antibody, Unconjugated(bsm-62460R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-62460R Image

Paraformaldehyde-fixed, paraffin embedded Human cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Acetyl-Histone H3.1 (Lys28) Monoclonal Antibody, Unconjugated(bsm-62460R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-62460R Image

Paraformaldehyde-fixed, paraffin embedded Human heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Acetyl-Histone H3.1 (Lys28) Monoclonal Antibody, Unconjugated(bsm-62460R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

BSM-62460R Image

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Acetyl-Histone H3.1 (Lys28) Monoclonal Antibody, Unconjugated(bsm-62460R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

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Manufacturer:	Bioss Inc
Shelf Life:	12 months
Type:	Recombinant Monoclonal
Alias:	Histone H3.1, Histone H3, HIST1H3A.
Shipping Condition:	Blue Ice
Unit(s):	100 ul, 20 ul
Host name:	Rabbit
Clone:
Isotype:	IgG
Immunogen:	KLH conjugated synthetic peptide derived from human Histone H3.1 around the acetylation site of K28
Application:	FC, ICC, IF, IHC-P, IP, ChIP, WB, IHC-Fr

Overview
Datasheets

Description

Description: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.