Akt (Ser-473), Phosphospecific Antibody

Catalogue Number: BSM-70330M-BSS

Manufacturer:	Bioss Inc
Type:	Monoclonal Primary Antibody - Unconjugated
Alias:	PKBalpha, PKB, AKT
Shipping Condition:	Blue Ice
Unit(s):	200 ul
Host name:	Mouse
Clone:	M114
Isotype:	IgG1
Immunogen:	Clone M114 was generated from a peptide containing amino acid residues surrounding Serine 473 in human Akt1. This sequence is highly conserved in human and mouse Akt, and may recognize Akt2 and Akt3.
Application:	IP, WB

Overview
Datasheets

Description

Description: Akt (PKB, Rac kinase) is a 60kDa ser/thr kinase critical for controlling diverse cellular functions, including glucose metabolism, gene transcription, cell proliferation, and apoptosis. Akt phosphorylates a number of substrates including MBP, glycogen synthetase, PKA RII subunit, and histone H1. Akt is activated in response to insulin and growth factors in a PI3-kinase dependent manner. Activation of PI3-Kinase generates phosphatidylinositol 3,4-bisphosphate, which induces membrane translocation of Akt coincident with its phosphorylation at Thr-308 and Ser-473. Upon activation, Akt associates with members of the PKC family of kinases, such as PKCδ and PKCζ. Ceramide-activated PKCζ leads to phosphorylation of Thr-34 within the pleckstrin homology domain of Akt. This phosphorylation inhibits PIP3 binding to Akt preventing activation of the kinase and may lead to cermide-induced cell death.

Additional Text

Gene Name

AKT1

Gene ID

207

Uniprot ID

P31749

Purification

Protein G purified

Modification

Phosphorylated, Ser473

Antibody Clonality

Monoclonal

Storage Note

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

BSM-70330M Datasheet

Price

£324.00

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