Argonaute 2 (N-terminus) Antibody
Catalogue Number: BSM-70340T-BSS
| Manufacturer: | Bioss Inc |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Ago2, Argonaute, eIF-2C2, eIF2C2; protein slicer |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rat |
| Clone: | 11A9 |
| Isotype: | IgG2a |
| Immunogen: | Clone 11A9 was generated from an Ago2 synthetic peptide (coupled to KLH) corresponding to amino acids in the N-terminus of human Ago2. This sequence has low homology to other argonaute family members. |
| Application: | WB |
Description
Description: Several classes of small RNAs, including short interfering RNAs (siRNAs), microRNAs (miRNAs), and Piwi-interacting RNAs (piRNAs) have been identified. MicroRNAs are about 21 nucleotides in length and have been implicated in many cellular processes such as development, differentiation, and stress response. These small RNAs function together with complexes called micro-ribonucleoproteins (miRNPs) to regulate gene expression by modulating mRNA translation or stability. Among the most important components in these complexes are argonaute proteins. There are four members in the mammalian argonaute family and only argonaute 2 (Ago2) possesses the Slicer endonuclease activity. Argonaute proteins participate in various steps of microRNA-mediated gene silencing, such as repression of translation and mRNA turnover. These activities may be regulated by cell signaling events that alter argonaute phosphorylation. EGFR phosphorylates Tyr-393 in Ago2, which reduces binding to Dicer and inhibits miRNA processing. Akt3 phosphorylates Ago2 at Ser-387 leading to reduced mRNA cleavage and enhanced translational repression.
Additional Text
Uniprot ID
Q9UKV8
Gene Name
Ago2
Gene ID
27161
Purification
Protein G purified
Antibody Clonality
Monoclonal
Modification
Unmodified
Storage Note
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
BSM-70340T Datasheet
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