mAbMods™ Anti-Mouse CD20 (Clone 18B12)

Catalogue Number: C1043-LET

Manufacturer:	Leinco Technologies, Inc
Type:	Recombinant Monoclonal
Shipping Condition:	Blue Ice
Unit(s):	1 mg, 25 mg, 5.0 mg, 50 mg
Host name:
Clone:	18B12
Isotype:
Immunogen:
Application:	FC, IF, WB, FA, Depl, InVivoA

Overview

Description

Description: This anti-CD20 antibody is a recombinant chimeric version of the original clone 18B12 antibody. The variable domain sequences are identical to the original clone 18B12; however, the constant region sequences have been switched from mouse IgG1 to mouse IgG2a. CD20 is a phosphoprotein belonging to the MS4A family, with a size of 33-37 kD. It is primarily found on B cells, including malignant B cells. This protein serves as a target, for therapies that aim to deplete B cells using antibodies 1-4. CD20 works alongside surface IgM and plays a role in calcium conductance, cell cycle progression, and B cell growth 5,6. Many B cell malignancies exhibit levels of CD20, which remain on the cell surface after treatment with monoclonal antibodies. This makes it an effective target for antibody-based treatments 7. Although CD20 is mainly associated with B cells it has also been observed on a subset of T lymphocytes and follicular dendritic cells. However, its function in these cell types is not yet fully understood. CD20-positive T cells have been detected in conditions like autoimmune diseases, cancers, and infections 8,9. Clone 18B12 is commonly used in research for studying B cell biology and immune responses. Researchers utilize this antibody for applications like flow cytometry analysis, immunohistochemistry studies as well as in vivo investigations using mouse models. By employing this antibody variant scientists have made progress in understanding the role of CD20, in autoimmune diseases, lymphomas, and other disorders related to B cell dysfunction 10-14.

Additional Text

Concentration

5 mg/ml

Antibody Clonality

Monoclonal, Recombinant Monoclonal

Storage Note

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Price

£208.00

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