Anti-Mouse CD29 (Clone KMI6) - Purified in vivo PLATINUM™ Functional Grade

Catalogue Number: C1102-LET

Manufacturer:	Leinco Technologies, Inc
Type:	Monoclonal Primary Antibody - Unconjugated
Shipping Condition:	Blue Ice
Unit(s):	1 mg, 100 mg, 25 mg, 5 mg, 50 mg
Host name:
Clone:	KMI6
Isotype:
Immunogen:
Application:	ELISA, FC, IF, IP, EM, WB, FA, NT

Overview

Description

Description: Stromal cells are needed for the early steps of B lymphocyte differentiation and proliferation1. CD29, also known as integrin beta 12, is a 110 kDa protein discovered due to its expression on bone marrow stromal cells, at cell surface areas that interact with undifferentiated lymphoid cells1. Integrin beta 1 is a member of a family of alpha beta heterodimers that act as cell surface receptors for extracellular matrix proteins3, including collagen, fibronectin, fibrinogen, laminin, VCAM1, and vitronectin among others4. CD29 is also involved in sperm-egg fusion. Through these interactions, CD29 regulates and/or promotes cell adhesion, laminin matrix deposition, endothelial cell motility, angiogenesis, osteoblast compaction, the formation of mineralized bone nodules, and cytokinesis. KMI6 was raised against the B cell-supportive stromal cell line BMS21. LOU/MN rats were immunized with BMS2 cells and subsequently spleen cells were fused with murine Sp2/0 for hybridoma generation. Antibody recognition is insensitive to the glycosylation state but is lost when the CD29 protein is reduced for Western blotting2. As such, the epitope is likely conformation-dependent. KMI6 was used to identify CD292. KMI6 can enhance cell adhesion of fibronectin to CD4 -8- thymocytes3.

Additional Text

Concentration

5 mg/ml

Purification

Purified

Antibody Clonality

Monoclonal

Storage Note

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Price

£200.00

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