Mouse anti Human cyclin E
Catalogue Number: C115M-NMB
| Manufacturer: | Nordic MuBio |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | G1/S-specific cyclin E1; CCNE1 |
| Shipping Condition: | RT |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | 168 |
| Isotype: | IgG2a |
| Immunogen: | Recombinant full-length human cyclin E protein |
| Application: | IP, WB |
Description
Description: Human cyclin E was originally identified by complementation of a triple cln deletion in Saccharomyces cerevisiae. Following the identification of human cyclin E protein, researchers found that breast cancers and some other solid tumors exhibited both quantitative and qualitative alterations in cyclin E protein production. In particular, the alterations in cyclin E expression in breast cancer correlated with increasing stage and grade of the tumor. These results suggested that cyclin E might be useful as a prognostic marker. More recently, Geng et al generated a mouse strain in which the coding sequences for cyclin D1 was deleted and replaced with those of human cyclin E. Replacement of cyclin D1 with cyclin E rescued all phenotypic manifestations seen in the cyclin D1 deficient mice and restored normal development to cyclin D1-dependent tissues. Based on the results of these studies, it appears that cyclin E is the major downstream target of cyclin D1. In addition to functioning downstream of cyclin D1, recent studies with Xenopus egg extracts demonstrated that cyclin E localizes to the centrosome. In these studies CDK2-cyclin E activity was shown to be required for centrosome duplication during S phase. Taken together, these data suggest a mechanism that coordinates centrosome reproduction with cycles of DNA synthesis and mitosis.
Additional Text
Gene ID
898
Gene Name
CCNE1
Uniprot ID
P24864
Purification
Protein A/G Purified
Antibody Clonality
Monoclonal
Application Notes
Antibody detects human cyclin E protein by Western blot. Detects a 50 kDa band. Optimal concentration should be evaluated by serial dilutions.
Storage Note
Product should be stored at -20C. Aliquot to avoid freeze/thaw cycles
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