Description

Description: CD3/CD4/CD8 combination is a fluorescent reagent containing mouse anti-human of CD3, CD4 and CD8 monoclonal antibodies conjugated to the tandem fluorochrome PE-Cy5, phycoerythrin, and fluorescein, respectively. This reagent permits the simultaneous identification of CD3+ mature T lymphocytes, CD4+ helper/inducer T lymphocytes and CD8+ suppressor/cytotoxic T lymphocytes in human peripheral blood by flow cytometric methods. CD3 monoclonal antibody recognizes the CD3 antigen. The monoclonal antibody may be used to enumerate mature T lymphocytes in human peripheral blood. This monoclonal antibody may also be used, in combination with other indicators, for the monoclonal antibody recognizes the CD3 antigen. May be used to enumerate mature T lymphocytes in human peripheral blood. May also be used, in combination with other indicators, for the diagnosis or prognosis of some immunodeficiency diseases, including agammaglobulinemia and the severe combined immunodeficiency disease (SCID), which exhibit decreased percentages of T lymphocytes. CD4 monoclonal antibody recognizes the CD4 antigen. This antibody may be used, in combination with other indicators, for the diagnosis or prognosis of immunodeficiency diseases, including hypogammaglobulinemia, severe combined immunodeficiency (SCID) and the acquired immunodeficiency disease (AIDS). The CD4 antigen domain is generally recognized to contain the binding site for the HIV retrovirus through interaction with the viral envelope glycoprotein. The major cellular or cell-derived elements of human peripheral blood include lymphocytes, monocytes, granulocytes, red blood cells and platelets. The mature lymphocyte population contains functionally distinct cell types that are referred to as T (thymus-derived), B and NK (Natural Killer) cells. Two major subsets of T lymphocytes have distinctly different functional properties, and can be identified by correspondingly different cell surface glycoprotein antigens (1). The CD3+ mature T lymphocytes expressing the CD4 surface antigen are responsible for helper-inducer activity, while CD3+ cells that express the CD8 antigen have suppressor and cytotoxic activity (2). CD3 is a complex consisting of at least five glycoprotein chains, each having a molecular weight of 20-25kD. The gamma subunit, and probably other subunits, of this molecule are closely associated with the alpha and beta chains of the T cell receptor (TCR) molecule (3). The CD3/TCR complex is responsible for the recognition of antigens which are expressed in association with the major histocompatibility (MHC) antigens. The CD3 molecule is generally understood to initiate or mediate T cell activation through signals derived from the TCR molecule (4). The CD3 molecule is present on the majority of resting and activated mature T lymphocytes, and has been used extensively to enumerate these cells in human peripheral blood. This molecule is also present on some natural killer (NK) cells. The CD3 molecule is a T cell lineage marker and is present on the majority of mature thymocytes. The expression of CD3 on thymocytes normally follows the expression of CD2, CD5 and CD7. CD4 monoclonal antibody recognizes the CD4 antigen (11). This antibody may be used, in combination with other indicators, for the diagnosis or prognosis of immunodeficiency diseases, including hypogammaglobulinemia, severe combined immunodeficiency (SCID) and the acquired immunodeficiency disease (AIDS) (14-16). The CD4 antigen domain is generally recognized to contain the binding site for the HIV retrovirus through interaction with the viral envelope glycoprotein (17). Infection of CD4+ T lymphocytes with the HIV virus eventually results in a profound depletion of these cells with the progression of disease. HIV Infected cells continue to serve as a reservoir for the replicating virus. A decrease in the population of CD4+ cells is directly correlated with immune deficit and deterioration in infected patients (18). CD8 monoclonal antibody recognizes the CD8 antigen (11). This antibody may be used, in combination with other indicators, for the diagnosis or prognosis of immunodeficiency diseases, including, severe combined immunodeficiency (SCID) and the acquired immunodeficiency disease (AIDS) (12-15). The CD8 monoclonal antibody may be useful in the identification of retroviral and other viral infections. An increase in the percentage of CD8+ cells has been observed in cytomegalovirus (CMV) and hepatitis B infections, as well in HIV disease (14-16).

Additional Text

Caution

FOR RESEARCH USE ONLY

Specificity

CD3, CD4, CD8

Purification

Purified

Antibody Clonality

Monoclonal