CD4 (CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Surface Glycoprotein CD4) (MaxLight 650)
Catalogue Number: C2255-27B-ML650-USB
| Manufacturer: | United States Biological |
| Physical state: | Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650. |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Alias: | CD4 antigen|p55|T-cell surface antigen T4/Leu-3|T-cell surface glycoprotein CD4|W3/25|W3/25 antigen |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | OX-35 |
| Isotype: | IgG2a |
| Immunogen: | MLR generated rat T helper lymphocytes. |
| Application: | FC, IHC |
Description
Description: MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. CD4, a single chain transmembrane glycoprotein is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lympocytes. It is also present on 80% of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV. Antibody to CD4 recognizes T-helper cells required for recognition of class II antigens. It reacts with 60% of peripheral blood E rosette-positive (E+) cells while showing negligible reactivity with E+ cells, monocytes, granulocytes, EBV-transformed B cell lines, and mouse splenocytes. CD4-positive T-cells provide helper funciton in pokeweed mitogen driven B-cell differentiation. Moreover, anti-CD4 selectively blocks the autologus mixed lymphocyte reaction (AMLR) with minimal or no effect on the allogeneic MLR (allo-MLR). Blocking of the autoreactivity occurs when either autologus B-lymphocytes or macrophages are used as stimulators. Specificity closely approximates the binding spectrum of OKT4 and Leu-3 antibodies both of which also recognize 55 to 65% of human peripheral blood T-cells.
Additional Text
Gene Name
CD4
Specificity
Recognizes the rat CD4 cell surface antigen, a 55kD glycoprotein expressed by helper T cells and weakly by monocytes.
Uniprot ID
P05540
Gene ID
24932
Purification
Protein A purified
Antibody Clonality
Monoclonal
C2255-27B-ML650 Datasheet
SUPPORT
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PRODUCT
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