CD4 (CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Surface Glycoprotein CD4) (MaxLight 650)

Catalogue Number: C2255-27W-ML650-USB

Manufacturer:	United States Biological
Physical state:	Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Type:	Monoclonal Primary Antibody - Conjugated
Shipping Condition:	Blue Ice
Unit(s):	100 ul
Host name:	Rat
Clone:	3H770 (YTS191.1)
Isotype:	IgG2b
Immunogen:
Application:	FC, IHC

Overview
Datasheets

Description

Description: MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. CD4, a single chain transmembrane glycoprotein is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lympocytes. It is also present on 80% of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV. Antibody to CD4 recognizes T-helper cells required for recognition of class II antigens. It reacts with 60% of peripheral blood E rosette-positive (E+) cells while showing negligible reactivity with E+ cells, monocytes, granulocytes, EBV-transformed B cell lines, and mouse splenocytes. CD4-positive T-cells provide helper funciton in pokeweed mitogen driven B-cell differentiation. Moreover, anti-CD4 selectively blocks the autologus mixed lymphocyte reaction (AMLR) with minimal or no effect on the allogeneic MLR (allo-MLR). Blocking of the autoreactivity occurs when either autologus B-lymphocytes or macrophages are used as stimulators. Specificity closely approximates the binding spectrum of OKT4 and Leu-3 antibodies both of which also recognize 55 to 65% of human peripheral blood T-cells.

Additional Text

Specificity

Recognizes the mouse CD4 cell surface antigen, expressed by a subset of T lymphocytes.

Purification

Protein G purified

Antibody Clonality

Monoclonal

C2255-27W-ML650 Datasheet

Price

£832.00

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Quantity
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