CD14 (MaxLight 550)
Catalogue Number: C2265-35A-ML550-USB
| Manufacturer: | United States Biological |
| Physical state: | Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550. |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | 3H504 (MEM-18) |
| Isotype: | IgG1 |
| Immunogen: | CD14 |
| Application: | IP, WB, FLISA |
Description
Description: MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000. The CD14 molecule is found predominantly on monocytes and macrophages in Flow Cytometry (Not Tested); it is less strongly expressed on granulocytes, and is absent from stem cells and myeloid cells of very early differentiation states. In immunohistochemistry, the CD14 molecule is found to be present on Langerhans cells, follicular dendritic cells, histocytes and high endothelial venules. In FLISA, recognizes the soluble form CD14. Reported to block the binding of bacterial lipopolysaccharide (LPS) to monocytes (2). Removal of sodium azide is recommended prior to use in functional assays.
Additional Text
Specificity
Antibody recognizes a monocyte surface antigen of 55kD classified as CD14.
Purification
Ion Exchange
Antibody Clonality
Monoclonal
C2265-35A-ML550 Datasheet
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