CD40 (PE/CY5)
Catalogue Number: C2392-17F-USB
| Manufacturer: | United States Biological |
| Physical state: | Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE/CY5. |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rat |
| Clone: | 5K254 (1C10) |
| Isotype: | IgG2a, kappa |
| Immunogen: | |
| Application: | FC, IF, IP |
Description
Description: CD40 is a type I cell surface protein belonging to the tumor necrosis factor superfamily of cell surface receptors. In mice it is expressed on B lineage cells, follicular dendritic cells, thymic epithelium, and interdigitating cells in the T-cell zone of secondary lymphoid organs.(1-5) CD40 first becomes detectable on a subset of small pre-B II cells in bone marrow, with the levels of CD40 expression increasing thereafter during B-cell maturation. Immature B cells (IgM+IgDloB220lo) express intermediate levels of CD40, whereas mature B cells (IgM+IgDhiB220hi) express high levels.4 CD40 has a central role in B cell growth and differentiation, and signalling through CD40 in combination with IL-4 reportedly induces immunoglobulin isotype switching and secretion of IgE.6 The agonistic monoclonal antibody closely resembles gp39/CD40 ligand in its ability to stimulate proliferation of small, resting B lymphocytes in the absence of other cofactors. PE/CY5 combination conjugate is used as a third fluorochrome in multicolor flow cytometry applications when only a single 488nm excitation source is available. Antibodies are covalently coupled using heterobifunctional linking reagents, resulting in an optimal conjugation format for flow cytometry.
Additional Text
Caution
FOR RESEARCH USE ONLY
Specificity
Recognizes mouse CD40, Mr 45-50kD.
Purification
Affinity Purified
Antibody Clonality
Monoclonal
C2392-17F Datasheet
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