CD90.2 (Thy1.2, Thy 1b) (MaxLight 650)
Catalogue Number: C2441-85B-ML650-USB
| Manufacturer: | United States Biological |
| Physical state: | Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650. |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rat |
| Clone: | FF-10 |
| Isotype: | IgG2c |
| Immunogen: | EL-4 cells. |
| Application: | IP, WB, IHC |
Description
Description: MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Thy-1 was discovered in early 1960s during the search for heterologous antisera against mouse leukemia cells. It was originally named theta (θ) antigen, then Thy-1 (THYmocyte differentiation antigen 1) due to its prior identification in thymocytes (precursors of T cells in the thymus). The human homolog was isolated in 1980 as a 25kDa protein (p25) of T-lymphoblastoid cell line MOLT-3 binding with anti-monkey-thymocyte antisera. The conserved gene and its alleles Thy-1 has been conserved throughout vertebrate evolution and even in some invertebrates, with homologs described in many species like squid, frogs, chickens, mice, rats, dogs, and humans. The Thy-1 gene is located at human chromosome 11q22.3 (mouse chromosome 9qA5.1). Some believe that there may be a functional significance of both this gene and CD3 delta subunit (T3D) mapping to chromosome 11q in man and chromosome 9 in mouse, though there is no homology (in fact this speculation lead to its localization in chromosome 11q-the human chromosome region syntenic to mouse chromosome 9 which harbored T3D). In mouse there are two alleles Thy1.1 (Thy 1a, CD90.1) and Thy1.2 (Thy 1b, CD90.2) which differ by one amino acid at position 108 (arginine in Thy-1.1 and glutamine in Thy-1.2). Thy 1.2 is expressed by most strains of mice, whereas Thy1.1 is expressed by some like AKR/J and PL mouse strains.
Additional Text
Gene Name
THY1
Gene ID
21838
Specificity
Recognizes the mouse Thy1.2 cell surface antigen and binds to Tcell lines (EL-4, 3A9), T lymphocytes and the WEH1-3 cell line. 3T3 fibroblasts are also stained by this antibody. This clone recognizes a 30kD glycoprotein in immunoprecipitation and Western blotting assays. The antibody strongly induces homotypic adhesion of Thy1.2 positive cell lines.
Purification
Protein G purified
Uniprot ID
P01831
Antibody Clonality
Monoclonal
C2441-85B-ML650 Datasheet
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