Anti-Mouse CD226 [Clone 10E5] - Purified in vivo GOLD™ Functional Grade
Catalogue Number: C2481-LET
| Manufacturer: | Leinco Technologies, Inc |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg, 100 mg, 25 mg, 5 mg, 50 mg |
| Application: | FC, FA, InVivoA |
Description
Description: Murine (m) CD226 (DNAM-1; PTA1) is an adhesion molecule involved in intercellular adhesion, lymphocyte signaling, cytotoxicity, and lymphokine secretion mediated by cytotoxic T- lymphocyte and natural killer (NK) cells (Uniprot Q8K4F0)1. mCD226 is also essential for NK- mediated killing of immature dendritic cells 2 and acts as an important costimulatory molecule in Th1 cell activation and effector function3. CD226 binds to PVR (CD155) via its N-terminal Ig-like domain2 and with cell surface receptor NECTIN2 (CD112)1. Based on its similarity to human CD226 (Uniprot Q15762), mCD226 likely competes with PVRIG for NECTIN2 binding. mCD226 is a marker for mature T cells2 and is specifically expressed on the surface of differentiated Th1 cells but not Th2 or Th0 cells3. mCD226 expression is enhanced by Th1 differentiation but down-regulated upon Th2 polarization. 10E5 was generated by immunizing Lewis and Lou/M rats with established murine Th1 cell clone AE7 and Th1 cell lines derived from 5B6 and DO11.10 TCR transgenic mice3. The resulting panel was screened on polarized Th1 and Th2 T cell clones and cell lines to find an antibody that selectively recognizes only Th1 clones. 10E5 specifically recognizes CD226 on the surface of Th1 cells. Additionally, 10E5 inhibits Ag-specific T cell expansion, reducing the expansion of PLP 139-151-specific Th1 cells producing IFN-γ. In vivo treatment with anti- CD226 clone 10E5 inhibits T cell activation and delays the onset and reduces the severity of Th1-mediated autoimmune disease.
Additional Text
Concentration
5 mg/ml
Purification
Purified
Antibody Clonality
Monoclonal
Storage Note
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
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