Anti-Mouse CD107b (LAMP-2/Mac-3) (Clone M3/84) - Purified No Carrier Protein

Catalogue Number: C2493-LET

Manufacturer:	Leinco Technologies, Inc
Type:	Monoclonal Primary Antibody - Unconjugated
Shipping Condition:	Blue Ice
Unit(s):	100 ug, 50 ug, 500 ug
Host name:
Clone:	M3/84
Isotype:
Immunogen:
Application:	FC, ICC, IP, RIA, IHC

Overview

Description

Description: Macrophages function in phagocytosis of foreign organisms and materials as well as resistance to tumors1. Additionally, they play roles in T lymphocyte immune responsiveness, inflammatory responses, and regulation of hematopoiesis. Lysosomes are the membrane-bound vacuoles that digest foreign materials and perform specialized autolytic functions2. Lysosomes also play a role in antigen presentation, cholesterol metabolism, and hydrolysis of exogenous materials3 . CD107b (LAMP-2, Mac-3) is a member of the lysosome-associated membrane protein (LAMP) family and is an important component of the lysosomal membrane involved in autophagy3,4 . CD107b is a highly glycosylated transmembrane protein, with glycosylation being necessary for its stability5,6. Mice deficient in CD107b have a severe phenotype, with 50% dying aged 20-40 days6. In humans, mutations in CD107b lead to Danon disease. The immunogen used to generate M3/84 was produced by first purifying surface glycoproteins from macrophage membranes of peritoneal exudate cells of C57BL/6 mice5. Next, previously recognized antigens were removed by immunoadsorbent columns to obtain a glycoprotein- enriched fraction. Then, (Lewis x BN) F1 rats were injected and splenocytes fused to mouse myeloma NS1 for hybridoma production.

Additional Text

Purification

Purified

Antibody Clonality

Monoclonal

Concentration

1 mg/ml

Storage Note

This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Price

£104.00

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