Anti-Mouse CD1d (CD1.1) - Purified in vivo GOLD™ Functional Grade
Catalogue Number: C2501-LET
| Manufacturer: | Leinco Technologies, Inc |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg, 100 mg, 25 mg, 5 mg, 50 mg |
| Host name: | |
| Clone: | 20H2 |
| Isotype: | |
| Immunogen: | |
| Application: | ELISA, FC, IP, WB |
Description
Description: CD1d is a non-classical major histocompatibility complex (MHC) molecule that presents glycolipid instead of peptide antigens1 . CD1d is non-MHC-encoded, heavily glycosylated, and a surface protein associated with β2-microglobulin2 . CD1d is present on invariant natural killer (iNKT) cells, where it elicits an immune response upon ligation with the T cell receptor and agonist α-galactosylceramide (α-GalCer )1. iNKT cells activated by α-GalCer secrete cytokines and undergo proliferation. Additionally, α-GalCer can induce anti-tumor immunity. CD1d can also interact with the iNKT agonist β-mannosylceramide (β-ManCer). 20H2 was generated by immunizing male Wistar rats with C57BL/6 thymocytes, boosting three times with a RBL-CD1.1-transfected rat cell line, injecting once with BALB/c thymocytes, and finally injecting with CR1-CD1.1 cells2 . The resulting spleen cells were fused with Sp2/0 cells for hybridoma production. 20H2 cross-reacts with CD1.2-transfected cells and binds to an epitope in the vicinity of the CD1d antigen-binding groove. 20H2 blocks T cell recognition of CD1d2 and can be used to block β-ManCer and α-GalCer agonistic activity1 . Blocking with 20H2 may also attenuate non-allergic asthma3 and ameliorate some aspects of neurodegenerative pathology in mouse4.
Additional Text
Concentration
5 mg/ml
Purification
Purified
Antibody Clonality
Monoclonal
Storage Note
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
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