Anti-Mouse CD1d (CD1.1) - Biotin
Catalogue Number: C2503-LET
| Manufacturer: | Leinco Technologies, Inc |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug, 500 ug |
| Host name: | |
| Clone: | 20H2 |
| Isotype: | |
| Immunogen: | |
| Application: | ELISA, FC, IP, WB |
Description
Description: CD1d is a non-classical major histocompatibility complex (MHC) molecule that presents glycolipid instead of peptide antigens1 . CD1d is non-MHC-encoded, heavily glycosylated, and a surface protein associated with β2-microglobulin2 . CD1d is present on invariant natural killer (iNKT) cells, where it elicits an immune response upon ligation with the T cell receptor and agonist α-galactosylceramide (α-GalCer )1. iNKT cells activated by α-GalCer secrete cytokines and undergo proliferation. Additionally, α-GalCer can induce anti-tumor immunity. CD1d can also interact with the iNKT agonist β-mannosylceramide (β-ManCer). 20H2 was generated by immunizing male Wistar rats with C57BL/6 thymocytes, boosting three times with a RBL-CD1.1-transfected rat cell line, injecting once with BALB/c thymocytes, and finally injecting with CR1-CD1.1 cells2 . The resulting spleen cells were fused with Sp2/0 cells for hybridoma production. 20H2 cross-reacts with CD1.2-transfected cells and binds to an epitope in the vicinity of the CD1d antigen-binding groove. 20H2 blocks T cell recognition of CD1d2 and can be used to block β-ManCer and α-GalCer agonistic activity1 . Blocking with 20H2 may also attenuate non-allergic asthma3 and ameliorate some aspects of neurodegenerative pathology in mouse4.
Additional Text
Concentration
0.5 mg/ml
Antibody Clonality
Monoclonal
Storage Note
This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations