Anti-Human CD25 (IL-2R) (Daclizumab) - Fc Muted™

Catalogue Number: C2515-LET

Manufacturer:	Leinco Technologies, Inc
Type:	Recombinant Monoclonal
Shipping Condition:	Blue Ice
Unit(s):	500 ug
Host name:
Clone:	Hu102
Isotype:
Immunogen:
Application:	ELISA, FC, WB, IHC

Overview

Description

Description: Interleukin-2 receptor (IL-2R), which regulates normal immune function 1 and is involved in signal transduction, cell growth and survival 2 , is composed of CD25, CD122, and CD132 3 . CD25 is the alpha-chain of IL-2R 2 and its expression is upregulated in resting T cells after activation, which in turn increases the binding capacity of IL-2 and positively affects signaling for T cell proliferation and survival 4. Daclizumab prevents the formation of the heterotrimeric IL-2R and selectively blocks IL-2R-mediated signaling 3 . By masking the IL-2 binding site on IL-2R, daclizumab inhibits T cell activation and proliferation as well as prevents IL-2 from stimulating Tregs to induce apoptosis in effector T cells 4. Additionally, daclizumab can remove CD25 from the surfaces of T cells via monocyte-dependent trogocytosis (defined as the active transfer of plasma membrane fragments between two live cells triggered by interaction between a cognate antigen on one cell and an antigen receptor signaling pathway on another cell) 3 . Daclizumab also inhibits activation and proliferation of T cells by blocking dendritic cells from presenting IL-2 to resting T cells 4 . Daclizumab reduces T cell CD25 levels via a mechanism that requires Fc domain interaction with FcR on monocytes, but not on natural killer cells 3 . Blocking IL-2 from binding to T cells leads to increased binding to CD56 bright NK cells via the IL-2R beta subunit 4 . This then leads to an expansion of CD56 bright NK cells, which target and kill activated T cells and is associated with reduced inflammation in the brain and decreased atrophy of brain tissue. Daclizumab is humanized anti-Tac 5, 6 and is composed of two humanized gamma-1 heavy chains and two humanized kappa light chains 4 that are sequence optimized for high affinity5, 6 . Daclizumab has been used in the treatment or prevention of a variety of autoimmune disorders and renal allograft rejection, respectively 6.

Additional Text

Concentration

5 mg/ml

Antibody Clonality

Monoclonal, Recombinant Monoclonal

Storage Note

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Price

£332.00

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