L-Leucine 4-methoxy-β-naphthylamide (hydrochloride)

Catalogue Number: C3708-APE

Manufacturer:	Apexbio
Molecular Weight:	322.8
Molecular Formula:	C17H22N2O2 HCl
Physical state:	A crystalline solid
Type:	Enzyme Substrates
Alias:	Leu-MNA
Shipping Condition:	Blue Ice

Overview
Datasheets

Description

Description: L-Leucine 4-methoxy-β-naphthylamide is a substrate for aminopeptidase M and leucine aminopeptidase.Aminopeptidases are enzymes catalyzing the cleavage of amino acids from the amino terminus (N-terminus) of proteins or peptides. Aminopeptidases are distributed widely throughout the animal and plant kingdoms and are present in various subcellular organelles, in cytosol, as well as membrane components.In vitro: L-Leucine 4-methoxy-β-naphthylamide was identified as a cell-permeable substrate for aminopeptidase M and leucine aminopeptidase that was developed for intracellular analysis of protease activities [1]. In a previous method-developing study, peptide derivatives of 4-methoxy-β-naphthylamine including L-leucine 4-methoxy-β-naphthylamide were incubated in microtiter plates together with nitrosalicylaldehyde. Selectivity was achieved by running parallel assays containing inhibitors partially selective for each of peptide derivatives. This method was successfully validated by measurements in cells isolated from cathepsin B-/--, K-/--, and L-/-- mice [2].In vivo: Up to now, there is no animal in vivo data reported.Clinical trial: So far, no clinical study has been conducted.References:[1] Monis, B.,Wasserkrug, H. and Seligman, A.M. Comparison of fixatives and substrates for aminopeptidase. Journal of Histochemistry and Cytochemistry 13(6), 503-509 (1965).[2] Ruettger, A.,Mollenhauer, J.,Lser, R., et al. Microplate assay for quantitative determination of cathepsin activities in viable cells using derivatives of 4-methoxy-β-naphthylamide. Biotechniques 41(4), 469-473 (2006).

Additional Text

Storage Note

Store at -20°C

CAS Number

4467-68-9

Smile String

COC1=CC(NC([C@H](CC(C)C)N)=O)=CC2=CC=CC=C21.Cl

C3708 CofA

C3708 MSDS

C3708 Datasheet

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