Connexin 43 (Cx43, Gap Junction Alpha 1 Protein, CxA-1) (MaxLight 550)

Catalogue Number: C7857-ML550-USB

Manufacturer:	United States Biological
Physical state:	Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Type:	Monoclonal Primary Antibody - Conjugated
Alias:	alpha 1 connexin\|AU042049\|AW546267\|Cnx43\|connexin43\|connexin-43\|Cx43\|Cx43alpha1\|gap junction 43 kDa heart protein\|gap junction alpha-1 protein\|gap junction membrane channel protein alpha 1\|Gja-1\|Npm1
Shipping Condition:	Blue Ice
Unit(s):	100 ul
Host name:	Mouse
Clone:	2Q250 (4E6.2)
Isotype:	IgG1
Immunogen:	Synthetic peptide corresponding to aa252-270 at the C-terminal of the mouse connexin 43 sequence. Species Sequence Homology: bovine
Application:	ICC, WB, IHC, FLISA

Overview
Datasheets

Description

Description: MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000. Mouse connexin 43 is a 382 amino acid gap junction protein with a predicted MW ~43kD. It is prominently expressed in heart. Gap junctions are composed of transmembrane channels that link the cytoplasm of neighboring cells. They differ from other membrane channels since they exist between two cells. Gap junctions are relatively non-specific and allow passive diffusion of small molecules up to 1000 Daltons. The junctions exist in almost all vertebrate and non-vertebrate cells. It is believed that gap junction play an important for intercellular communications and affect growth and differentiation of cells. Gap junctional channel is composed of a hemichannel (connexon) in the cell membrane of one cell joined in mirror symmetry with a connexon in the opposing cell. Each connexon is an oligomer of six protein subunits that define the axial aqueous pore. Molecular cloning studies have identified a family of at least 12 highly related Connexins that are designated according to mol. wt, Cx26-50. Hydropathy analyses of Cx sequences predicts 4 transmembrane, 2 extracellular (EC), and 3 cytoplasmic (CP) domains. The EC, TM, and N-terminal CP domains are well conserved among family members, while Central and C-terminal domains are highly variable in both sequence and size. The N and C-termini are predicted to be cytoplasmic.

Additional Text

Gene Name

GJA1

Specificity

Recognizes mouse Connexin 43, Mr 43-47kD. Species Crossreactivity: porcine, rat, human and canine. Species Sequence Homology: bovine. No known homology with other connexins.

Purification

Protein A purified

Gene ID

14609

Uniprot ID

P23242

Antibody Clonality

Monoclonal

C7857-ML550 Datasheet

Price

£1,070.00

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