Biotinylated Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (Primary Amine Labeling) (CD3-HM157B)
Catalogue Number: CD3-HM157B-KAT
CD3-HM157B Image
CD3-HM157B Image
CD3-HM157B Image
CD3-HM157B Image
The purity of Biotinylated Human CD3E&CD3G is greater than 95% as determined by SEC-HPLC.
CD3-HM157B Image
Biotinylated Human CD3E&CD3G on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
CD3-HM157B Image
Immobilized Biotinylated Human CD3E&CD3G, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD3 Antibody, mFc Tag with the EC50 of 64.5ng/ml determined by ELISA (QC Test).
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 17.9 kDa (CD3E) and 17.9 kDa (CD3G). Due to glycosylation, the protein migrates to 25-40 kDa based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Protein - Conjugated |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3E & CD3G , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Additional Text
Protein Tag
HIS Tag
Uniprot ID
P07766, P09693
Gene ID
916; 917
Gene Name
CD3E, CD3G
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
Immobilized Anti-CD3 Antibody, mFc Tag at 5Oeg/ml (100Oel/well) on the plate. Dose response curve for Biotinylated Human CD3E&CD3G, His Tag with the EC50 of 0.43Oeg/ml determined by ELISA. See testing image for detail.
Endotoxin
Less than 1 EU per µg by the LAL method.
Short Description
Recombinant Biotinylated Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus of CD3G.It contains Asp23-Asp126 (CD3E) and Gln23-Ser116 (CD3G).
Specificity
CD3; CD3 epsilon; CD3epsilon; CD3ε; CD3 ε; CD3E; CD3e; CD3 gamma; CD3gamma; CD3γ; CD3 γ; CD3G; CD3g; CD3epsilon&CD3gamma; CD3 epsilon&CD3 gamma; CD3 epsilon & CD3 gamma; CD3ε&CD3γ; CD3 ε&CD3 γ; CD3 ε & CD3 γ; CD3E&CD3G; CD3E & CD3G; CD3 E & CD3 G; CD3e&CD3g; CD3e & CD3g; CD3 e & CD3 g
CD3-HM157B Datasheet
SUPPORT
outstanding technical support
PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations