Biotinylated Human CD30/TNFRSF8 Protein (CD3-HM430B)
Catalogue Number: CD3-HM430B-KAT
CD3-HM430B Image
CD3-HM430B Image
CD3-HM430B Image
CD3-HM430B Image
CD3-HM430B Image
The purity of Biotinylated CD30 is greater than 95% as determined by SEC-HPLC.
CD3-HM430B Image
Biotinylated CD30 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
CD3-HM430B Image
Immobilized Human CD30 Ligand, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD30, His Tag with the EC50 of 17.5ng/ml determined by ELISA (QC Test).
CD3-HM430B Image
Immobilized Anti-CD30 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD30, His Tag with the EC50 of 11.5ng/ml determined by ELISA.
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 41.3 kDa. Due to glycosylation, the protein migrates to 70-100 kDa based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Protein - Conjugated |
| Alias: | CD30; CD30KI-1; CD30L receptor; TNFRSF8; D1S166EKi-1; CD30KI-1 |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: The transmembrane receptor CD30 (TNFRSF8) and its ligand CD30L (CD153, TNFSF8) are members of the tumor necrosis factor (TNF) superfamily and display restricted expression in subpopulations of activated T-and B-cells in nonpathologic conditions. CD30 expression is upregulated in various hematological malignancies, including Reed-Sternberg cells in Hodgkin's disease (HD), anaplastic large cell lymphoma (ALCL) and subsets of Non-Hodgkin's lymphomas (NHLs).
Additional Text
Protein Tag
HIS-AviTag™
Short Description
Recombinant Biotinylated Human CD30/TNFRSF8 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe19-Lys379.
UniProt ID
P28908-1
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
Immobilized Anti-CD30 Antibody, hFc Tag at 0.5Oeg/ml (100Oel/well) on the plate. Dose response curve for Biotinylated Human CD30, His Tag with the EC50 of 49ng/ml determined by ELISA. See testing image for detail.
Endotoxin
Less than 1 EU per µg by the LAL method.
CD3-HM430B Datasheet
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