Biotinylated Human Siglec-3/CD33 Protein (CD3-HM433B)
Catalogue Number: CD3-HM433B-KAT
CD3-HM433B Image
CD3-HM433B Image
CD3-HM433B Image
CD3-HM433B Image
Biotinylated Human Siglec-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
CD3-HM433B Image
Immobilized Biotinylated Human Siglec-3, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.
CD3-HM433B Image
The purity of Biotinylated Human Siglec-3 is greater than 95% as determined by SEC-HPLC.
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 29.6 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Bis-Tris PAGE result. |
| Physical state: | Liquid |
| Type: | Protein - Conjugated |
| Alias: | CD33 molecule; CD33; FLJ00391; gp67; Siglec3; Siglec-3; p67 |
| Host Cell: | HEK293 cells |
| Shipping Condition: | Dry Ice |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state.They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11. To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs.
Additional Text
Protein Tag
HIS-AviTag™
Short Description
Recombinant Biotinylated Human Siglec-3/CD33 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp18-His259.
Storage Note
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
UniProt ID
P20138-1
Biological Activity
Immobilized Anti-Siglec-3 Antibody, hFc Tag at 0.5Oeg/ml (100Oel/well) on the plate. Dose response curve for Biotinylated Human Siglec-3, His Tag with the EC50 of 40.9ng/ml determined by ELISA. See testing image for detail.
Endotoxin
Less than 1 EU per µg by the LAL method.
CD3-HM433B Datasheet
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