Human CD40/TNFRSF5 Protein (CD4-HM240)
Catalogue Number: CD4-HM240-KAT
CD4-HM240 Image
CD4-HM240 Image
CD4-HM240 Image
CD4-HM240 Image
CD4-HM240 Image
The purity of Human CD40 is greater than 95% as determined by SEC-HPLC.
CD4-HM240 Image
Human CD40 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
CD4-HM240 Image
Immobilized Human CD40 Ligand Trimer, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 101.8ng/ml determined by ELISA.
CD4-HM240 Image
Loaded Human CD40, hFc Tag on ProA-Biosensor can bind Human CD40 Ligand (Trimer), His-Flag Tag with an affinity constant of 0.23 µM as determined in BLI assay (Gator® Prime).
| Manufacturer: | Kactus |
| Shelf Life: | 24 months |
| Molecular Weight: | The protein has a predicted MW of 45.9 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Other Recombinant Protein |
| Alias: | CD40; CD40L receptor; TNFRSF5; CD40 antigen; CD40 molecule; CDw40; MGC9013 |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects.CD40 molecule is a potential target for cancer immunotherapy. There are number of completed and ongoing clinical trials where agonistic anti-CD40 monoclonal antibodies are employed to activate an anti-tumor T cell response via activation of dendritic cells.
Additional Text
Protein Tag
hFC Tag
Uniprot ID
P25942-1
Short Description
Recombinant Human CD40/TNFRSF5 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Glu21-Arg193.
Storage Note
-20 to -80°C for 24 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
Immobilized Human CD40 Ligand Trimer, His Tag at 1Oeg/ml (100Oel/Well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 0.42Oeg/ml determined by ELISA. See testing image for detail.
Endotoxin
Less than 1 EU per µg by the LAL method.
CD4-HM240 Datasheet
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