Biotinylated Human CD40/TNFRSF5 Protein (CD4-HM440B)
Catalogue Number: CD4-HM440B-KAT
CD4-HM440B Image
CD4-HM440B Image
CD4-HM440B Image
CD4-HM440B Image
CD4-HM440B Image
CD4-HM440B Image
Serial dilutions of Anti-CD40 Antibody were added into Biotinylated Human CD40, His Tag : Human CD40 Ligand (Trimer) , hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 2.0ug/ml.
CD4-HM440B Image
The purity of Biotinylated Human CD40 is greater than 95% as determined by SEC-HPLC.
CD4-HM440B Image
Biotinylated Human CD40 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
CD4-HM440B Image
Immobilized Biotinylated Human CD40, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD40 Ligand (Trimer), hFc Tag with the EC50 of 9.5ng/ml determined by ELISA (QC test).
CD4-HM440B Image
Immobilized Anti-CD40 Antibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD40, His Tag with the EC50 of 31.3ng/ml determined by ELISA.
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 22.1 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Protein - Conjugated |
| Alias: | CD40; CD40L receptor; TNFRSF5; CD40 antigen; CD40 molecule; CDw40; MGC9013 |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects.CD40 molecule is a potential target for cancer immunotherapy. There are number of completed and ongoing clinical trials where agonistic anti-CD40 monoclonal antibodies are employed to activate an anti-tumor T cell response via activation of dendritic cells.
Additional Text
Gene Name
CD40
Gene ID
958
Uniprot ID
P25942
Protein Tag
HIS-AviTag™
Short Description
Recombinant Biotinylated Human CD40/TNFRSF5 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu21-Arg193.
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
Immobilized Biotinylated Human CD40, His Tag at 1Oeg/ml (100Oel/Well) on the plate. Dose response curve for Human CD40L, hFc Tag with the EC50 of 33ng/ml determined by ELISA. See testing image for detail.
Endotoxin
Less than 1 EU per µg by the LAL method.
CD4-HM440B Datasheet
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