Mouse CD40/TNFRSF5 Protein (CD4-MM140)
Catalogue Number: CD4-MM140-KAT
CD4-MM140 Image
CD4-MM140 Image
CD4-MM140 Image
CD4-MM140 Image
CD4-MM140 Image
Mouse CD40, His Tag immobilized on CM5 Chip can bind Mouse CD40 Ligand, His Tag with an affinity constant of 0.54 nM as determined in SPR assay (Biacore T200).
CD4-MM140 Image
The purity of Mouse CD40 is greater than 95% as determined by SEC-HPLC.
CD4-MM140 Image
Mouse CD40 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
CD4-MM140 Image
Immobilized Mouse CD40, His Tag at 2ug/ml (100 ul/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 3.5 ng/ml determined by ELISA (QC Test).
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 19.9 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Other Recombinant Protein |
| Alias: | CD40; CD40L receptor; TNFRSF5; CD40 antigen; CD40 molecule; CDw40; MGC9013 |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects.CD40 molecule is a potential target for cancer immunotherapy. There are number of completed and ongoing clinical trials where agonistic anti-CD40 monoclonal antibodies are employed to activate an anti-tumor T cell response via activation of dendritic cells.
Additional Text
Protein Tag
HIS Tag
Uniprot ID
P27512-1
Short Description
Recombinant Mouse CD40/TNFRSF5 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val24-Arg193.
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
Immobilized Mouse CD40, His Tag at 1Oeg/ml (100Oel/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 0.12Oeg/ml determined by ELISA. See testing image for detail.
Endotoxin
Less than 1 EU per µg by the LAL method.
CD4-MM140 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations