Mouse IL-2 R alpha/CD25 Protein (CD5-MM125)
Catalogue Number: CD5-MM125-KAT
CD5-MM125 Image
CD5-MM125 Image
CD5-MM125 Image
CD5-MM125 Image
CD5-MM125 Image
Mouse IL-2 R alpha, His Tag immobilized on CM5 Chip can bind Mouse IL-2, His Tag with an affinity constant of 30.49 nM as determined in SPR assay (Biacore T200).
CD5-MM125 Image
The purity of Mouse IL-2 R alpha is greater than 95% as determined by SEC-HPLC.
CD5-MM125 Image
Mouse IL-2 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
CD5-MM125 Image
Immobilized Mouse IL-2 R alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 0.15ug/ml determined by ELISA (QC Test).
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 25.76 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Other Recombinant Protein |
| Alias: | CD25; IDDM10; IL-2 R alpha; IL2R; IL2RA; p55; TCGFR |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL-2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
Additional Text
Gene Name
IL2RA
Gene ID
16184
Uniprot ID
P01590
Protein Tag
HIS Tag
Short Description
Recombinant Mouse IL-2 R alpha/CD25 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu22-Lys236.
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
Immobilized Mouse IL-2 R alpha, His Tag at 2Oeg/ml (100Oel/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 95.8ng/ml determined by ELISA. The affinity constant of 30.49 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Endotoxin
Less than 1 EU per µg by the LAL method.
CD5-MM125 Datasheet
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