Human CD47 Protein (CD7-HM147)
Catalogue Number: CD7-HM147-KAT
CD7-HM147 Image
CD7-HM147 Image
CD7-HM147 Image
CD7-HM147 Image
CD7-HM147 Image
Human CD47, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human SIRP alpha, hFc Tag with an affinity constant of 18.9nM as determined in SPR assay (Biacore T200).
CD7-HM147 Image
Human CD47 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
CD7-HM147 Image
The purity of Human CD47 is greater than 95% as determined by SEC-HPLC.
CD7-HM147 Image
Immobilized Human CD47, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 82.1ng/ml determined by ELISA (QC Test).
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 14.5 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Other Recombinant Protein |
| Alias: | CD47 glycoprotein; CD47 molecule; CD47; IAP; OA3; MER6 |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers.
Additional Text
Protein Tag
HIS Tag
Short Description
Recombinant Human CD47 is expressed from HEK293 with His tag at the C-Terminus.It contains Gln19-Pro139.
UniProt ID
Q08722-1
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
Immobilized Human CD47, His Tag at 2Oeg/ml (100Oel/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 46.8ng/ml determined by ELISA. The affinity constant of 18.9nM as determined in SPR assay (Biacore T200). See testing image for detail.
Endotoxin
Less than 1 EU per µg by the LAL method.
CD7-HM147 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations