Human CD73/NT5E Protein (CD7-HM173)
Catalogue Number: CD7-HM173-KAT
CD7-HM173 Image
CD7-HM173 Image
CD7-HM173 Image
CD7-HM173 Image
CD7-HM173 Image
Immobilized Human CD73, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test).
CD7-HM173 Image
Human CD73 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
CD7-HM173 Image
Loaded Anti-CD73 Antibody, hFc Tag on ProA-Biosensor can bind Human CD73, His Tag with an affinity constant of 0.31 nM as determined in BLI assay (Gator® Prime).
CD7-HM173 Image
Measured-by-its-ability-to-hydrolyze-the-5'-phosphate-group-from-the-substrate-adenosine-5'-monophosphate- (AMP). The specific activity is >15000 pmol/min/ug (QC Test).
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 58.8 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Other Enzymes |
| Alias: | CD73; NT5E; NTE; 5' nucleotidase (CD73); 5'-NT; 5-NT; E5NT; EC 3.1.3.5; eN; eNT; NT; NT55'-nucleotidase; NT5; NTE; CALJA |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: CD73, also known as ecto-5'-nucleotidase, is an enzyme that in humans is encoded by the NT5E gene.CD73 commonly serves to convert AMP to adenosine.The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. A deficiency of CD73 occurs in a variety of immunodeficiency diseases.
Additional Text
Protein Tag
HIS Tag
Short Description
Recombinant Human CD73/NT5E Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Trp27-Lys547.
UniProt ID
P21589-1
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
Immobilized Human CD73, His Tag at 0.5Oeg/ml (100Oel/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 17.2ng/ml determined by ELISA. See testing image for detail.
Endotoxin
Less than 1 EU per µg by the LAL method.
CD7-HM173 Datasheet
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