Biotinylated Human CD47 Protein (CD7-HM447B)
Catalogue Number: CD7-HM447B-KAT
CD7-HM447B Image
CD7-HM447B Image
CD7-HM447B Image
CD7-HM447B Image
The purity of Biotinylated Human CD47 is greater than 95% as determined by SEC-HPLC.
CD7-HM447B Image
Biotinylated Human CD47 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
CD7-HM447B Image
Immobilized Biotinylated Human CD47, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA.
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 18.38 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Protein - Conjugated |
| Alias: | CD47 glycoprotein; CD47 molecule; CD47; IAP; OA3; MER6 |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers.
Additional Text
Protein Tag
HIS-AviTag™
Short Description
Recombinant Biotinylated Human CD47 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains GIn19-Pro139.
UniProt ID
Q08722-1
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
Immobilized Human SIRP alpha, hFc Tag at 1Oeg/ml (100Oel/Well) on the plate. Dose response curve for Biotinylated Human CD47, His Tag with the EC50 of 0.48Oeg/ml determined by ELISA. See testing image for detail.
Endotoxin
Less than 1 EU per µg by the LAL method.
CD7-HM447B Datasheet
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