Mouse CD47 Protein (CD7-MM247)
Catalogue Number: CD7-MM247-KAT
CD7-MM247 Image
CD7-MM247 Image
CD7-MM247 Image
CD7-MM247 Image
CD7-MM247 Image
Mouse CD47, hFc Tag captured on CM5 Chip via Protein A can bind Mouse SIRP alpha, His Tag with an affinity constant of 28.33 nM as determined in SPR assay (Biacore T200).
CD7-MM247 Image
The purity of Mouse CD47 is greater than 95% as determined by SEC-HPLC.
CD7-MM247 Image
Mouse CD47 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
CD7-MM247 Image
Immobilized Mouse SIRP alpha, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD47, hFc Tag with the EC50 of 38.6ng/ml determined by ELISA (QC Test).
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 40.60 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Other Recombinant Protein |
| Alias: | CD47 glycoprotein; CD47 molecule; CD47; IAP; OA3; MER6 |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers.
Additional Text
Protein Tag
hFC Tag
Uniprot ID
Q61735-1
Short Description
Recombinant Mouse CD47 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln19-Lys140.
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
The affinity constant of 28.33 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Endotoxin
Less than 1 EU per µg by the LAL method.
CD7-MM247 Datasheet
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