Biotinylated Human CD8 alpha/CD8A Protein (CD8-HM48AB)
Catalogue Number: CD8-HM48AB-KAT
CD8-HM48AB Image
CD8-HM48AB Image
CD8-HM48AB Image
Biotinylated Human CD8 alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
CD8-HM48AB Image
Immobilized Biotinylated Human CD8 alpha, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test).
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 20.5 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Protein - Conjugated |
| Alias: | CD8a; Lyt-2; CD8; Leu2; MAL; p32; CD8a molecule; OKT8 T-cell antigen |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: CD8 alpha enhances the responses of antigen-specific CTL activated through TCR through binding MHC class I, favoring lipid raft partitioning of TCR, and inducing intracellular signaling. CD8 alpha is also found on dendritic cells and rat macrophages, but whether CD8 alpha enhances responses of a partner receptor, like TCR, to activate these cells is not known.
Additional Text
Protein Tag
HIS-AviTag™
Short Description
Recombinant Biotinylated Human CD8 alpha/CD8A Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser22-Asp182.
UniProt ID
P01732-1
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
Immobilized Anti-CD8 Antibody, hFc Tag at 0.5Oeg/ml (100Oel/well) on the plate. Dose response curve for Biotinylated Human CD8 alpha, His Tag with the EC50 of 6.0ng/ml determined by ELISA. See testing image for detail.
Endotoxin
Less than 1 EU per µg by the LAL method.
CD8-HM48AB Datasheet
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