Biotinylated Human CD40 Ligand/TNFSF5 Trimer Protein (Primary Amine Labeling) (CDL-HM140B)
Catalogue Number: CDL-HM140B-KAT
CDL-HM140B Image
CDL-HM140B Image
CDL-HM140B Image
CDL-HM140B Image
The purity of Biotinylated Human CD40 Ligand Trimer is greater than 95% as determined by SEC-HPLC.
CDL-HM140B Image
Biotinylated Human CD40 Ligand Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
CDL-HM140B Image
Immobilized Human CD40, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD40 Ligand Trimer, His Tag with the EC50 of 47.6ng/ml determined by ELISA (QC Test).
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 52.4 kDa, Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Protein Ligand |
| Alias: | CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.
Additional Text
Uniprot ID
P29965
Gene Name
CD40LG
Gene ID
959
Protein Tag
HIS-FLAG™ Tag
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
Immobilized Biotinylated Human CD40 Ligand Trimer, His Tag at 2Oeg/ml (100Oel/well) on the streptavidin precoated plate (5Oeg/ml). Dose response curve for Human CD40, hFc Tag with the EC50 of 0.77Oeg/ml determined by ELISA. See testing image for detail.
Endotoxin
Less than 1 EU per µg by the LAL method.
Short Description
Recombinant Biotinylated Human CD40 Ligand/TNFSF5 Trimer Protein (Primary Amine Labeling) is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Met113-Leu261 trimer design.
CDL-HM140B Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations