Biotinylated Human CD27 Ligand/CD70 Trimer Protein (Primary Amine Labeling) (CDL-HM427B)
Catalogue Number: CDL-HM427B-KAT
CDL-HM427B Image
CDL-HM427B Image
CDL-HM427B Image
CDL-HM427B Image
The purity of Biotinylated Human CD27 Ligand (Trimer) is greater than 95% as determined by SEC-HPLC.
CDL-HM427B Image
Biotinylated Human CD27 Ligand (Trimer) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
CDL-HM427B Image
Immobilized Human CD27, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human CD27 Ligand (Trimer), His Tag with the EC50 of 12.1ng/ml determined by ELISA (QC Test).
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 51.8 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result. |
| Physical state: | Lyophilized |
| Type: | Cytokines |
| Alias: | CD70 molecule; CD70; TNFSF7; CD27 Ligand; CD27-L; CD27LG; Ki-24 antigen; TNFSF7G |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: CD70, also named CD27 ligand (CD27L), is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7. CD70 is a cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.
Additional Text
Protein Tag
HIS Tag
UniProt ID
P32970-1
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biological Activity
Immobilized Biotinylated Human CD27 Ligand (Trimer) , His Tag at 2Oeg/ml (100Oel/Well) on the plate. Dose response curve for Human CD27, hFc Tag with the EC50 67ng/ml determined by ELISA. See testing image for detail.
Endotoxin
Less than 1 EU per µg by the LAL method.
Short Description
Recombinant Biotinylated Human CD27 Ligand/CD70 Trimer Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the N-Terminus.It contains Leu50-Pro193 trimer design.
CDL-HM427B Datasheet
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