5-Vinyl-uridine (5-VU), 5-Ethenyl-uridine
Catalogue Number: CLK-049-50-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Physical state: | solid |
| Type: | Conjugation Reagent |
| Shipping Condition: | RT |
| Unit(s): | 50 mg |
Description
Description: 5-VU (5-Vinyl-uridine) can potentially be used as a replacement for BrU (5-Bromo-uridine) or the copper-catalyst requiring 5-EU (5-Ethynyl-uridine) to measure de novo RNA synthesis in proliferating cells. 5-VU is cell permeable and might incorporate into nascent RNA instead of its natural analog uridine. This assumption is based on the previously demonstrated suitability of 5-VdU (5-Vinyl-2'-deoxyuridine) for DNA synthesis monitoring[1]. The resulting vinyl-functionalized RNA could subsequently be detected via Cu(I)-free Alkene-Tetrazine Ligation that offers the choice to introduce a Biotin group (via Tetrazines of Biotin) for subsequent purification tasks or a fluorescent group (via Tetrazines of fluorescent dyes) for subsequent microscopic imaging.
Additional Text
Solubility
DMSO, methanol
Application Notes
RNA synthesis monitoring (potentially)
Smile String
n1(cc(c(=O)[nH]c1=O)C=C)[C@H]1[C@@H]([C@@H]([C@H](O1)CO)O)O
CLK-049-50 MSDS
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