Catalogue Number: CLK-050-10-JEN
Manufacturer: | Jena Bioscience |
Shelf Life: | 12 months |
Physical state: | solid |
Type: | Conjugation Reagent |
Shipping Condition: | RT |
Unit(s): | 10 mg |
Description: 5-VdU (5-Vinyl-2'-deoxyuridine) can be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine) or the copper-catalyst requiring 5-EdU (5-Ethynyl-2'-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle. 5-VdU is cell permeable and incorporates into replicating DNA instead of its natural analog thymidine. The resulting vinyl-functionalized DNA can subsequently be detected via Cu(I)-free Alkene-Tetrazine Ligation that offers the choice to introduce a Biotin group (via Tetrazines of Biotin) for subsequent purification tasks or a fluorescent group (via Tetrazines of fluorescent dyes) for subsequent microscopic imaging [1].
DMSO, methanol
DNA synthesis monitoring[1]
2'-Deoxy-5-vinyluridine 2'-Deoxy-5-ethenyluridine
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