The CuAAC Biomolecule Reaction Buffer Kit (BTTAA based) is suitable to perform Copper (Cu(I))-catalyzed Azide-Alkyne Click chemistry reactions (CuAAC) with Azide- or Alkyne- modified biomolecules.
1 Kit provides sufficient amounts to perform 25 CuAAC experiments à 200 μl using 2 mM CuSO4 (copper source), 10 mM BTTAA (Cu(I)-stabilizing ligand) and 100 mM Na-Ascorbate (reduction reagent) in 100 mM Na-Phosphate reaction buffer.