6-Azido-Trehalose, 6-TreAz
Catalogue Number: CLK-078-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Physical state: | solid |
| Type: | Conjugation Reagent |
| Shipping Condition: | RT |
| Unit(s): | 1 mg |
Description
Description: Myobacteria are characterized by a trehalose-lipopolysaccharide-containing outer cell membrane. The Azide-functionalized 6-Azido-Trehalose provides an attractive approach for the detection of Myobacteria in living cultures: It is cell-permeable, intracellularly processed and incorporated instead of its natural counterpart. The resulting Azide-functionalized lipopolysaccharides can subsequently be detected via Cu(I)-catalyzed or Cu(I)-free Click Chemistry that offers the choice to introduce a Biotin group (via Alkynes of Biotin or DBCO-containing Biotin, respectively) for subsequent purification tasks or to introduce fluorescent group (via Alkynes of fluorescent dyes or DBCO-containing fluorescent dyes, respectively) for subsequent microscopic imaging. Myobacteria strains tested[1]: Mycobacterium smegmatis mc2155, Mycobacterium tuberculosis H37Rv,Mycobacterium bovis BCG
Additional Text
Solubility
water, DMSO
Application Notes
Metabolic Lipopolysaccharide labeling of Myobacteria
Smile String
[C@H]1([C@H]([C@H](O[C@@H]([C@H]1O)CN=[N+]=[N-])O[C@@H]1[C@@H]([C@H]([C@@H]([C@H](O1)CO)O)O)O)O)O
CLK-078 MSDS
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