Alkyne Agarose

Catalogue Number: CLK-1032-2-JEN

Manufacturer:Jena Bioscience
Shelf Life:12 months after date of delivery
Physical state:Suspension
Type:Agarose
Shipping Condition:RT
Unit(s): 2 ml

Additional Text

Additional Storage Information

do not freeze

Application

Alkyne Agarose is an efficient matrix to covalently capture CLICK-functionalized proteins by a Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC). The proteins of interest need to be metabolically, enzymatically or chemically azide-tagged. Subsequently, the alkyne agarose containing the covalently attached proteins can be washed with high stringency, virtually eliminating any non-specifically bound proteins. For a comprehensive protocol for Cu(I)-catalyzed purification of azide-tagged proteins, please refer to our Click Chemistry Capture Kit (CLK-1065).

Application Notes

Alkyne Agarose is an efficient matrix to covalently capture CLICK-functionalized proteins by a Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC). The proteins of interest need to be metabolically, enzymatically or chemically azide-tagged. Subsequently, the alkyne agarose containing the covalently attached proteins can be washed with high stringency, virtually eliminating any non-specifically bound proteins. For a comprehensive protocol for Cu(I)-catalyzed purification of azide-tagged proteins, please refer to our Click Chemistry Capture Kit (CLK-1065).

Reagent:Smile String

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