5-Ethynyl-2'-deoxyuridine (5-EdU)

Catalogue Number: CLK-N001-5000-JEN

Manufacturer:	Jena Bioscience
Shelf Life:	12 months
Physical state:	solid
Type:	Conjugation Reagent
Shipping Condition:	RT
Unit(s):	5 g

Overview
Datasheets

Description

Description: 5-EdU (5-Ethynyl-2'-deoxyuridine) can be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle. 5-EdU is cell permeable and incorporates into replicating DNA instead of its natural analog thymidine. The resulting ethynyl-functionalized DNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging [1-5]. Presolski et al.[6] and Hong et al.[7] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.

Additional Text

Solubility

DMSO, up to 200 mM (at room temperature) in 1 x PBS or water by heating the obtained suspension for 1 minute by 70 °C

Application Notes

DNA synthesis monitoring[1-5]

Smile String

n1(cc(c(=O)[nH]c1=O)C#C)[C@H]1C[C@@H]([C@H](O1)CO)O

Short Description

5-Ethynyl-2'-deoxyuridine

CLK-N001-5000 MSDS

CLK-N001-5000 Datasheet

Price

£3,044.00

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