5-Ethynyl-uridine (5-EU)
Catalogue Number: CLK-N002-10-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Physical state: | solid |
| Type: | Conjugation Reagent |
| Shipping Condition: | RT |
| Unit(s): | 10 mg |
Description
Description: Ethynyl-labeled uridine (5-EU) can be used as a replacement for BrU (5-Bromo-uridine) to measure de novo RNA synthesis in proliferating cells. 5-EU is cell permeable and incorporates into nascent RNA instead of its natural analog uridine. The resulting ethynyl-functionalized RNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging[1]. Presolski et al.[2] and Hong et al.[3] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.
Additional Text
Solubility
Up to 200 mM in 1 x PBS or water by heating the obtained suspension for 1 minute by 70 °C, less soluble in DMSO
Application Notes
RNA synthesis monitoring[1]
Short Description
5-Ethynyl-uridine
Smile String
n1(cc(c(=O)[nH]c1=O)C#C)[C@H]1[C@@H]([C@@H]([C@H](O1)CO)O)O
Additional Information
Long term storage at < -20°C. If stored as recommended, Jena Bioscience guarantees optimal performance of this product for 12 months after date of delivery.
CLK-N002-10 MSDS
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