2-Ethynyl-adenosine
Catalogue Number: CLK-N005-5-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Physical state: | solid |
| Type: | Conjugation Reagent |
| Shipping Condition: | RT |
| Unit(s): | 5 mg |
Description
Description: 2-Ethynyl-labeled adenosine (2-EA) can be used to measure de novo mRNA poly(A) tail synthesis in proliferating cells. 2-EA is cell permeable and incorporates into nascent mRNA transcripts both transcriptionally by RNA polymerase I,II and III and posttranscriptionally by poly(A) polymerase instead of their natural analolg adenosine. The resulting ethynyl-functionalized RNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging[1]. Presolski et al.[2] and Hong et al.[3] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.
Additional Text
Solubility
up to 4.0 mM in 1 x PBS
Application Notes
mRNA poly(A) tail synthesis monitoring[1]
Smile String
c12c(nc(nc2n(cn1)[C@H]1[C@@H]([C@@H]([C@H](O1)CO)O)O)C#C)N
CLK-N005-5 MSDS
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