C8-Alkyne-dCTP
Catalogue Number: CLK-T06-XL-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Ph: | 7.5 |
| Physical state: | Solution |
| Type: | Nucleotides |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ul |
| Application: | Cnjg |
Additional Text
Concentration
100 mM
Application Notes
Incorporation into DNA by PCR with family B polymerases (Pwo, Deep Vent exo- or KOD XL)[1,2] Incorporation into DNA by Primer Extension with family A polymerase Taq and family B polymerases (Pwo, Deep Vent exo- or KOD XL)[1] Incorporation into DNA by 3'-End Labeling with Terminal deoxynucleotidyl Transferase(TdT)[3] The resulting ethynyl-functionalized DNA can subsequently be processed via Cu(I)-catalyzed click chemistry that offers the choiceto introduce a Biotin group (via Azides of Biotin) for subsequent purification tasksto introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imagingto crosslink the DNA to Azide-functionalized biomolecules e.g.proteins Presolski et al.[4] and Hong et al.[5] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.
Short Description
5-(Octa-1,7-diynyl)-2'-deoxycytidine 5'-triphosphate, Sodium salt
Smile String
c1(nc(=O)n(cc1C#CCCCCC#C)[C@H]1C[C@H](O)[C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)N
Molecular Weight
571.31 g/mol (free acid)
Molecular Formula
C17H24N3O13P3 (free acid)
CLK-T06-XL MSDS
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