5-Ethynyl-dUTP (5-EdUTP)
Catalogue Number: CLK-T07-S-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Ph: | 7.5 +/-0 |
| Physical state: | Solution |
| Type: | Conjugation Reagent |
| Shipping Condition: | Blue Ice |
| Unit(s): | 5 ul |
| Application: | Cnjg |
Additional Text
Concentration
100-110 mM
Application Notes
Incorporation into DNA by PCR with family B polymerases (Pwo, Deep Vent exo- or KOD XL)[1,2] Incorporation into DNA by Primer Extension with family A polymerase Taq and family B polymerases (Pwo, Deep Vent exo- or KOD XL)[1] The resulting ethynyl-functionalized DNA can subsequently be processed via Cu(I)-catalyzed click chemistry that offers the choiceto introduce a Biotin group (via Azides of Biotin) for subsequent purification tasksto introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imagingto crosslink the DNA to Azide-functionalized biomolecules e.g.proteins Presolski et al.[3] and Hong et al.[4] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.
Short Description
5-Ethynyl-2'-deoxyuridine 5'-triphosphate, Sodium salt
Smile String
c1(=O)[nH]c(=O)n(cc1C#C)[C@H]1C[C@H](O)[C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O
CLK-T07-S MSDS
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