5-Ethynyl-UTP (5-EUTP)

Catalogue Number: CLK-T08-XL-JEN

Manufacturer:	Jena Bioscience
Shelf Life:	12 months
Ph:	7.5 +/-0
Physical state:	Solution
Type:	Conjugation Reagent
Shipping Condition:	Blue Ice
Unit(s):	50 ul
Application:	Cnjg

Overview
Datasheets

Additional Text

Concentration

100-110 mM

Application Notes

Incorporation into RNA by T7 RNA polymerase-mediated in vitro transcription. The resulting alkyne-functionalized RNA can subsequently be processed via Cu(I)-catalyzed (azide-alkyne) click chemistry that offers the choiceto introduce a Biotin group for subsequent purification tasks (via Azides of Biotin)to introduce fluorescent group for subsequent microscopic imaging (via Azides of fluorescent dyes)to crosslink the RNA to azide-functionalized biomolecules e.g.proteins Presolski et al.[1] and Hong et al.[2] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.

Short Description

5-Ethynyl-uridine-5'-triphosphate, Sodium salt

Smile String

n1([C@@H]2O[C@H](COP(=O)(OP(=O)(OP(=O)(O)O)O)O)[C@H]([C@H]2O)O)c(=O)[nH]c(=O)c(c1)C#C

CLK-T08-XL MSDS

CLK-T08-XL Datasheet

Price

£942.00

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